产品货号：
GS4582
中文名称：
重组大鼠FGF-21
英文名称：
Recombinant Rat Fibroblast Growth Factor-21
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
General Description
Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity.In mice,brown adipose tissue becomes a source of systemic FGF-21 after cold exposure.FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB.Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin.In addition,rat FGF-21 respectively shows 81% and 92% a.a.identity to human and murine FGF-21,and it show activity on murine and human cells.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 19.7kDa,a single non-glycosylated polypeptide chain containing 180 amino acids.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/mL,corresponding to a specific activity of> 2.0×103 IU/mg in the presence of 5μg/mL of rMuKlotho-β and 10μg/mL of heparin.
Physical Appearance
White lyophilized powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,500mM NaCl,pH7.4.
Endotoxin
Less than 1EU/μg of rRtFGF-21 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
相关搜索：重组大鼠FGF-21，大鼠FGF-21重组蛋白，Recombinant Rat Fibroblast Growth Factor-21
Fibroblast growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF family and it is specifically induced by HMGCS2 activity.In mice,brown adipose tissue becomes a source of systemic FGF-21 after cold exposure.FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB.Recombinant rat FGF-21 contains 180 amino acids residues and show limited binding to heparin.In addition,rat FGF-21 respectively shows 81% and 92% a.a.identity to human and murine FGF-21,and it show activity on murine and human cells.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 19.7kDa,a single non-glycosylated polypeptide chain containing 180 amino acids.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/mL,corresponding to a specific activity of> 2.0×103 IU/mg in the presence of 5μg/mL of rMuKlotho-β and 10μg/mL of heparin.
Physical Appearance
White lyophilized powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,500mM NaCl,pH7.4.
Endotoxin
Less than 1EU/μg of rRtFGF-21 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
相关搜索：重组大鼠FGF-21，大鼠FGF-21重组蛋白，Recombinant Rat Fibroblast Growth Factor-21